miR-145 is required for hepatic outgrowth.

Abstract

<p>A WISH assay was used for determination of the liver developmental markers <i>hhex</i> at 30 hpf (A-C, dorsal view), <i>sePb</i> at 50 hpf (D-F, dorsal views), <i>prox1</i> at 72 hpf (G-I, dorsal views) and <i>fabp10</i> at 96 hpf (J-L, dorsal views) in control, miR-145 inhibitor-injected and miR-145 mimic-injected embryos. The arrowhead indicates liver. The expression levels of <i>hhex</i>, <i>sePb</i>, <i>prox1</i> and <i>fabp10</i> were examined using qPCR (M). The <i>ef1a</i> expression level was served as an internal control (*P < 0.05, t-test). Twenty whole embryos per experimental group were used and three independent replicates were performed.</p