<p><b>A)</b> The function of unit sequencing cost of fragments was calculated by plotting sequencing depth versus fragment counts. The ORP was defined as the minimum value of the unit sequencing cost (the minimum value of the black-dashed line). <b>B)</b> The sequencing reads of three individuals were sampled at five thresholds (10%, 20%, 50%, 80%, and 100%, respectively). The sequencing depth (green) was equal to the good barcode read numbers divided by the fragment counts. The SRP was the corresponding good barcode reads when the slope of the fragment counts (orange curve) reduced to zero.</p
