Regulation of activin and follistatin expression by local release of GH in carp pituitary cells.

Abstract

<p>(A) GH treatment on activin βA, activin βB and follistatin mRNA expression at pituitary level. Pituitary cells were challenged for 24 hr with increasing concentrations of porcine GH as indicated. (B) Removal of endogenous GH by immunoneutralization on activin βA, activin βB and follistatin mRNA expression in carp pituitary cells. Pituitary cells were incubated for 24 hr with increasing levels of antiserum raised against carp GH and parallel treatment with normal rabbit serum (NRS, 1:2500) was used as a negative control. In these experiments, total RNA was isolated after drug treatment and used for real-time PCR measurement of activin βA, activin βB and follistatin mRNA, respectively. Data presented are pooled results from four separate experiments and individual groups denoted by different letters represent a significant different at P < 0.05 (ANOVA followed by Newman-Keuls test).</p

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