Regulation of activin and follistatin expression by local release of LH in carp pituitary cells.

Abstract

<p>(A) LH and (B) FSH treatment on activin βA, activin βB and follistatin mRNA expression at pituitary level. Pituitary cells were treated for 24 hr with increasing levels of equine LH and FSH as indicated. (C) Removal of endogenous LH by immunoneutralization on activin βA, activin βB and follistatin mRNA expression in carp pituitary cells. Pituitary cells were incubated for 24 hr with increasing levels of antiserum raised against carp LH and parallel treatment with NRS (1:5000) was used as a negative control. In these experiments, total RNA was isolated after drug treatment and used for real-time PCR measurement of activin βA, activin βB and follistatin mRNA, respectively. Data presented are pooled results from four experiments and individual groups denoted by different letters represent a significant different at P < 0.05 (ANOVA followed by Newman-Keuls test).</p