<p>a. Charged amino acids of five epitopes on HA included, Epitope A labeled in blue (124, 133, 135, 142 and 145), B in red (155, 156, 158, 160, 189, 190, 193 and 197), C in green (50, 53, 275, 276, 278, 279, 307 and 310), D in yellow (172, 173, 201, 207, 208, 222, 225 and 246) and E in cyan (57, 62, 63, 75, 82, 83, 92 and 261)(a.i and a-ii). b. The charged amino acids on epitopes of variants/vaccine strains were labeled, acidic ones in red, basic ones in blue and both acid and basic ones in green. c. T<sub>135</sub>K on Epitope A resulted in a decrease of NGT<sub>133-135</sub>, labeled in blue (T<sub>135</sub>K) and cyan and K<sub>160</sub>T on Epitope B did in an increase of NYT<sub>158-160</sub>, labeled in red (K<sub>160</sub>T) and cyan. Other amino acids on epitopes adjacent to NGS were labeled in green.</p
