Real-time diffusion of metabolites across a membrane.
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Abstract
<p>One side of each co-culture chamber was inoculated with <i>E</i>. <i>coli</i>, as seen in the pictorial legend on the right, each box represents a chamber with a black dashed line representing the membrane. The terms ‘pre-mixed’ and ‘gradient’ describe the initial media conditions. The gradient condition was loaded with LB on one side and 1x DPBS on the other. The pre-mixed condition was loaded with LB that was diluted in half with DPBS to simulate complete diffusion of LB across the membrane. These two conditions were tested with four initial concentrations of LB, 25%, 50%, 75%, and 100%, all diluted using 1x DPBS. The final pre-mixed concentration of the medium for each well was 12.5%, 25%, 37.5%, and 50% LB. The maximum and minimum values of the generated growth curves, conducted in triplicate (n = 3), are displayed as shaded regions around the plotted averages. This experiment was cultured as described in the methods for 24 hours.</p