STING degradation occurs in HDCs but not in HEK293.

Abstract

<p>(A) Western blotting assessment of STING and RIG-I in MRC-5, 2BS, KMB17 and hUC-MSC cells after transfection with 0.5 μg of pcDNA3.1 for 24 h. (B) MRC-5 and HEK293 cells were transfected with 0.5 μg of pcDNA3.1 for 24 hours. The indicated molecules were assessed by western blotting. (C, D) qRT-PCR assessment of mRNA levels (C) and ELISA assessment of protein levels (D) of IL-6 in MRC-5 and HEK293 cells after transfection of pcDNA3.1. (E) ULK1 S556 phosphorylation was assessed by western blotting in MRC-5 and HEK293 cells. Asterisk (*), P <0.05.</p