Responses of <i>At-MMPs</i> upon <i>Botrytis cinerea</i> infection.

Abstract

<p>(A) Expression profiles of <i>At-MMPs</i> in Arabidopsis accession Col-0 (WT) leaves in response to <i>Botrytis cinerea</i>. Five-week-old plants were inoculated with the fungus by placing 5 μl of spore suspension (2x10⁵ spores/ml in potato dextrose broth [PDB]) on the center of the rosette leaves. Mock treatment was performed with PDB. Total RNA was extracted from leaves at the indicated time points and used for RT-PCR. UBQ5 was used as an internal control. Experiments were independently repeated three times with similar results. (B) Molecular phylogenetic analysis of MMPs in Arabidopsis. The evolutionary history was inferred by Neighbor-Joining method using a conserved region spanning the cysteine switch and Met-turn motif of AtMMP protein sequences.</p