Structure and subcellular localization of At2-MMP.

Abstract

<p>(<b>A</b>) Domain structure of an At2-MMP-GFP fusion construct. Numbers denote the position of amino acids (aa). SP: signal peptide; Pro: propeptide domain; CYS: cysteine switch; F: predicted furin-cleavage site; CAT: catalytic domain; ZBD: zinc-binding domain; G: putative GPI-anchor modification site; TM: transmembrane domain. The <i>Sal</i>I site (GTCGAC, aa 337–338) is downstream of the zinc-binding domain and upstream of the GPI-anchor modification site. The GFP coding sequence was integrated at the <i>Sal</i>I site. (<b>B</b>) Subcellular localization of At2-MMP upon transiently expression of 35S::At2-MMP2-GFP together with 35S::mCherry in Arabidopsis WT leaves. At2-MMP-GFP was detected 24 h after bombardment. Left panel: without plasmolysis; middle and right panel: five min after plasmolysis induced with 50% glycerol. Bar = 20 μm.</p