Embryonic expression of zebrafish <i>b3glct</i> genes.

Abstract

<p>(A) RT-PCR analysis of <i>b3glct</i> expression demonstrates robust expression of both <i>b3glcta</i> (left panel) and <i>b3glctb</i> (middle panel) at different stages of development in whole embryos as well as various embryonic tissues at 48-hpf (right panel). Controls included <i>pitx2c</i> as negative control for 0-hpf, <i>rhodopsin</i> as negative control for the lens, <i>beta-actin</i> as positive control for all tissues and H₂O as negative contamination control for all reactions. (B) In-situ hybridization analysis of <i>b3glcta</i> and <i>b3glctb</i> expression demonstrates broad expression in 24-120-hpf embryos with enrichment in the developing eyes, fins, brain, craniofacial region and somites. aer–apical ectodermal ridge, ase–anterior segment of the eye, b–brain, cmz–ciliary marginal zone, crc–craniofacial cartilage, e–eye, f–fins, h–heart, le–lens, sm–skeletal muscles.</p