Decreased proliferation of immature neutrophils in <i>MiR125a</i>^<i>-/-</i> mice.

Abstract

<p>(A) Flow cytometry analysis of three subpopulations in CD11b⁺Gr-1⁺ neutrophils in bone marrow. Mature neutrophils (mNeu) indicate CD11b^hi Gr-1^hi cells. Immature neutrophils (imNeu) indicate CD11b^lowGr-1^hi cells and promyelocytes/myelocytes (pro/mye) indicate CD11b^intGr-1^int cells. The bar graph shows the average numbers of these subpopulations in <i>MiR125a</i>^<i>+/+</i> and <i>MiR125a</i>^<i>-/-</i> mice. Values were represented as mean±s.d., n = 5 mice of each genotype. (B) Flow cytometry analysis of three populations of CD11b⁺Gr-1⁺ neutrophils incorporating BrdU in bone marrow after in vivo pulsing BrdU for 72 hours. The bar graph indicates the average percentage of intensities of BrdU-incorporating cells (mean±s.d., n = 5). Ns, none significant difference, *<i>P</i><0.05, ***<i>P</i><0.001 (Student’s <i>t</i>-test).</p