RSV symptoms and miRNAs induced by NS3 in rice.

Abstract

<p>(A) Images of whole plants exhibiting stunted phenotypes. Scale bar = 15 cm. (B) Detection of the RSV <i>CP</i> gene by RT-PCR. (C) Images of RSV-infected leaves exhibiting chlorotic mottling and rolled-leaf phenotypes. Scale bar = 5 cm. (D) Detection of miRNA (miR168, 395, 398, 399, and 528) accumulations in healthy (mock-infected) and RSV-infected rice plants by northern blotting. Red star, 21-nt miR528. (E) Northern and western blot assay detection of miRNA (miR168 and miR395) accumulations and protein expression in mock-infected, RSV-infected (RSV), <i>NS2</i> OX#1, <i>NS3</i> OX#1, <i>SP</i> OX#1, and <i>NSvc4</i> OX#1 rice plants. In (D and E), U6 served as a loading control, the expression levels in the WT-Mock plants are set to a value of 1.0 and the expression levels in the other plants are relative to this reference value.</p