Additional file 2: Figure S2. PCR and phenotypic analysis of the Δcre1 strain T. reesei SDC11. a PCR analysis of T. reesei SDC11 with SP4 as control. 1 and 2 represent the fragment (upstream region and open reading frame of gene cre1) amplificated by the prime pair cre1-2426UF/cre1-1069R in T. reesei SDC11 and SP4, respectively; 3 and 4 represent the internal fragment of gene cre1 amplificated by the prime pair cre1-497F/cre1-1069R in T. reesei SDC11 and SP4, respectively. 5 and 6 represent the fragment of gene pyrG amplificated by the prime pair pyrG-UF1/pyrG-2426DR in T. reesei SDC11 and SCP11, respectively. b Southern blot analysis of the genomic DNA isolated from SP4 and SCP11, which were digested with EcoRI/HindIII. A 5.5-kb fragment is present in the parental strain SP4, and a 7.0-kb band is shown in Δcre1 + pyrG strain SCP11. c Growth of T. reesei SN1, Δcre1 + pyrG strain SCP11 and Δcre1 strain SDC11 on MM plate. d Growth of T. reesei SP4, Δcre1 + pyrG strain SCP11 and Δcre1 strain SDC11 on the MM plate containing uracil (0.1%)
