Hyperoxia upregulates MHC class I expression equally in 2D and 3D cultures.
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Abstract
<p><b>(A-D):</b> MCA205 tumors were grown as 2D monolayers <b>(A, C)</b> or as 3D spheroids <b>(B, D)</b> at 21% O<sub>2</sub> or 60% O<sub>2</sub> for 48h. MHC class I levels were determined by flow cytometry. The magnitude of MHC class I upregulation was similar in 2D and 3D cultures. Representative histograms (<b>A, B</b>) and associated quantification and statistics (<b>C, D</b>) of 4 independent experiments shown. The significance of differences was analyzed by the Student’s t-test (two-sided); p = 0.002 (C), p = 0.001 (D). Grey filled: Unstained control; Blue: Normoxia (21% O<sub>2</sub>); Green: Hyperoxia (60% O<sub>2</sub>). Error bars indicate SD.</p
