Additional file 5: Figure S5. of Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy

Abstract

Susceptible cells are indistinguishable from untreated cells before drug treatment. Distribution of fluorescence levels in the susceptible phenotype before drug treatment (t = 0) and in untreated control cells before regrowth in LB (t = 0) in the a) tolC, b) tnaC, and c) ptsG reporter strain. The two populations are not statistically different, an unpaired t test with Welch’s correction yielding a P value of 0.07, 0.7, and 0.9, respectively. The bottom and top of the box are the first and third quartiles, the band inside the box is the median, the bottom and top whiskers represent the 10th and 90th percentiles, respectively. Data are obtained at least in biological triplicate (N = 3) for each reporter strain employed for a total of n S  = 6659 and n C  = 3076 susceptible and control cells, respectively. We did not observe any significant difference between the results obtained from different biological replica. (PNG 423 kb

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