15 décembre 19361936/12/15 (A16,N5808)-1936/12/15

Arthur Ragauskas (19610)

Cassandra Collins (1296645)

Chang Yoo (3436814)

Gerald Tuskan (471216)

Jin-Gui Chen (52742)

Kimberly Winkeler (3859228)

Lee Gunter (88923)

Maud Hinchee (4722060)

Nancy Engle (3436811)

Sara Jawdy (3533078)

Timothy Tschaplinski (3436805)

Xiaohan Yang (430728)

Yongil Yang (3533084)

Yunqiao Pu (678767)

Biotechnology for Biofuels

Additional file 3. The gene copy number of PdDUF231A in OXPdDUF231A transgenic plants

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MOESM3 of Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

Confédération générale du travail (France). Auteur du texte

Gallica, Bibliotheque Numerique

Le Peuple : organe quotidien du syndicalisme

Additional file 4. Fold change and relative metabolite concentrations (Âľg/g FW sorbitol equivalents) of OXPdDUF231A transgenic plants versus empty vector control (K-147) Populus deltoides âWV94â leaves (LPI 5) of 6-month-old greenhouse-grown plants. Metabolites were analyzed as trimethylsilyl derivatives by gas chromatography-mass spectrometry. Data are the average (Avg) and standard error of the mean (sem) of six OXPdDUF231A plants (three plants from each of two independent transgenic lines) and three K-147 control plants. Unknown and partially identified metabolites are designated by retention time, key mass-to-charge (m/z) ratios, and identified moieties

MOESM4 of Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

Abstract Background Domain of Unknown Function 231-containing proteins (DUF231) are plant specific and their function is largely unknown. Studies in the model plants Arabidopsis and rice suggested that some DUF231 proteins act in the process of O-acetyl substitution of hemicellulose and esterification of pectin. However, little is known about the function of DUF231 proteins in woody plant species. Results This study provides evidence supporting that one member of DUF231 family proteins in the woody perennial plant Populus deltoides (genotype WV94), PdDUF231A, has a role in the acetylation of xylan and affects cellulose biosynthesis. A total of 52 DUF231-containing proteins were identified in the Populus genome. In P. deltoides transgenic lines overexpressing PdDUF231A (OXPdDUF231A), glucose and cellulose contents were increased. Consistent with these results, the transcript levels of cellulose biosynthesis-related genes were increased in the OXPdDUF231A transgenic lines. Furthermore, the relative content of total acetylated xylan was increased in the OXPdDUF231A transgenic lines. Enzymatic saccharification assays revealed that the rate of glucose release increased in OXPdDUF231A transgenic lines. Plant biomass productivity was also increased in OXPdDUF231A transgenic lines. Conclusions These results suggest that PdDUF231A affects cellulose biosynthesis and plays a role in the acetylation of xylan. PdDUF231A is a promising target for genetic modification for biofuel production because biomass productivity and compositional quality can be simultaneously improved through overexpression

Yongil Yang

Chang Geun Yoo

Kimberly A. Winkeler

Cassandra M. Collins

Maud A. W. Hinchee

Sara S. Jawdy

Lee E. Gunter

Nancy L. Engle

Yunqiao Pu

Xiaohan Yang

Timothy J. Tschaplinski

Arthur J. Ragauskas

Gerald A. Tuskan

Jin-Gui Chen

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Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

http://gallica.bnf.fr/ark:/12148/cb34348990g/date

Le Peuple : organe quotidien du syndicalisme

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