Ge Wang (56838)

Hui Liu (33624)

Jing Zhang (23775)

Jinshui Zhu (4428487)

Lidan Hou (4428493)

Rui Zhang (13940)

Xiaoyu Chen (298872)

Yanxia Huang (4428490)

Journal of Experimental & Clinical Cancer Research

Correlation of lncRNA AK023391 expression with clinicopathologic features of GC patients. (DOCX 25Â kb

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Additional file 3: Table S3. of LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway

Clinicopathological data of GC patients. (DOCX 25Â kb

Additional file 1: Table S1. of LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway

KaplanâMeier analysis of the correlation of AK023391 expression with survival in late stage patients. (TIFF 172Â kb

Additional file 4: of LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway

Abstract Background Patients with gastric cancer commonly have a poor prognosis, owing to its invasiveness and distant metastasis. Recent studies have confirmed the pivotal role of long non-coding RNAs (lncRNAs) in tumorigenesis and the progression of malignant tumors, including gastric cancer. However, little is known about the molecular mechanism by which lncRNA AK023391 contributes to gastric cancer. Methods A lncRNA microarray was used to identify the differentially expressed lncRNA AK023391 in gastric cancer and adjacent normal tissues. In addition, RNA fluorescence in situ hybridization (FISH) was used to investigate the association between AK023391 expression and the clinicopathological characteristics and prognosis of patients with gastric cancer. Subsequently, a series of in vitro assays and a xenograft tumor model were used to observe the functions of lncRNA AK023391 in gastric cancer cells. A cancer pathway microarray, bioinformatic analysis, western blotting, and immunochemistry were carried out to verify the regulation of AK023391 and its downstream PI3K/Akt signaling pathway. Results Expression of lncRNA AK023391 was significantly upregulated in gastric cancer samples and cell lines in comparison to adjacent normal tissues, and was positively correlated with poor survival in patients with gastric cancer. The multivariate Cox regression model revealed that AK023391 expression acted as an independent prognostic factor for survival in patients with gastric cancer. Knockdown of AK023391 inhibited cell growth and invasion both in vitro and in vivo, and induced apoptosis and cell cycle arrest in gastric cancer cells, whereas its overexpression reversed these effects. Mechanistically, PI3K/Akt signaling mediated the NF-κB, FOXO3a, and p53 pathways. Moreover, downstream transcription factors, such as c-myb, cyclinB1/G2, and BCL-6 might be involved in AK023391-induced tumorigenesis in gastric cancer. Conclusions The novel oncogenic lncRNA AK023391 in gastric cancer exerts its effects through activation of the PI3K/Akt signaling pathway, and may act as a potential biomarker for survival in patients with gastric cancer

Yanxia Huang

Jing Zhang

Lidan Hou

Ge Wang

Hui Liu

Rui Zhang

Xiaoyu Chen

Jinshui Zhu

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LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway

https://figshare.com/articles/Additional_file_1_Table_S1_of_LncRNA_AK023391_promotes_tumorigenesis_and_invasion_of_gastric_cancer_through_activation_of_the_PI3K_Akt_signaling_pathway/5739060

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