Quantification of IAA production in DC3000 <i>ald</i> mutants.

Abstract

<p>A) Measurement of IAA accumulation in supernatants of DC3000 <i>ald</i> single mutants grown for 48 hrs in HSC media supplemented with 0.25 mM IAAld. B) Growth of <i>ald</i> mutants in HSC media supplemented with 0.25 mM IAAld. These cultures were used for quantification of IAA shown in panel A. C) Measurement of IAA accumulation in supernatants of <i>aldA</i>, <i>aldB</i> and the <i>aldA aldB</i> double mutant grown for 48 hrs in HSC media supplemented with 0.25 mM IAAld. The <i>aldB</i> mutant in this experiment is the <i>aldB</i>::pJP5603Tet mutant. For panels A-C, values are an average of three biological replicates ± SEM (error bars too small to see in panel B). Letters indicate significant difference between samples within a given time point (<i>p</i><0.01. c* in panel A indicates that IAA levels in <i>aldB</i> are significantly different from wt at p<0.05).</p

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