Heterologous expression of putative DC3000 aldehyde dehydrogenases in <i>E</i>. <i>coli</i>.

Abstract

<p>DC3000 genes encoding putative aldehyde dehydrogenase proteins were expressed in <i>E</i>. <i>coli</i> BL21(DE3) cells. A) Quantification of IAA produced by strains expressing PSPTO_0728, PSPTO_2673, and PSPTO_3644 and pET-21a as a negative control. B) Quantification of IAA produced by strains expressing PSPTO_0092, PSPTO_3064, and PSPTO_3323. PSPTO_2673 was included as a control for comparison to panel A. IAA levels were measured in supernatants 24 hrs post-induction with addition of 0.25 mM IAAld. Values are an average ± SEM (n = 3). Letters indicate significant difference between samples within a given time point (<i>p</i><0.01). Similar results were obtained from two additional independent experiments.</p

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