Tup1 depletion bypasses the requirement for Wor1 in the expression of <i>WOR1</i> and key opaque regulators.

Abstract

<p><b>(A)</b> Expression levels in the conditional Tup1 mutant and <i>wor1 pMET3-tup1</i> conditional double mutant haploid strains. <i>pMET3-TUP1</i> (HLY4533) and <i>pMET3-TUP1 wor1</i> (HLY4539) were grown at room temperature for 48hr in the presence or absence of methionine. <b>(B)</b> Expression levels in diploid <i>wor1</i> and <i>wor1 tup1</i> mutant strains. Overnight cultures of <i>MTL</i><b><i>a</i></b><i>/</i><b><i>a</i></b> <i>wor1</i> (HLY3570), <i>wor1 tup1</i> (HLY4540), and a control strain (JYC1) were inoculated into fresh SCD and grown to log phase. Expression levels of the indicated genes in <b>(A)</b> and <b>(B)</b> were measured by qPCR and normalized to <i>ACT1</i>. <i>WOR1</i> expression was measured by qPCR using primers specific to either the <i>WOR1</i> 5’ UTR or the <i>WOR1</i> coding region (CDS). Average expression level of three independent qPCR experiments was plotted with error bars representing the s.d.<b>(C)</b> Genetic model of Wor1-Tup1 regulation of <i>WOR1</i> expression.</p

    Similar works

    Full text

    thumbnail-image

    Available Versions