DS2 enhances agonist binding in δ-subunit containing receptors.


<p>The δ-subunit specific modulator, DS2, modulates [<sup>3</sup>H]muscimol binding (2 nM) in α4β3N-Flag-δ GABA<sub>A</sub>Rs. For the membranes, the data are the mean and standard deviation of two experiments, and for micelle reconstituted receptors for a single experiment in triplicate. The curves were fitted by nonlinear least squares to the Hill equation. The EC<sub>50</sub> (μM), Hill coefficient and maximum modulation were: for membranes, 2.0 ± 0.7 μM, 0.9 ± 0.2, 139 ± 4%; for reconstituted 2.3 ± 0.8 μM, 1.2 ± 0.5, 132 ± 4%.</p

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