Braun, Mihály

Sárkány-Kiss, Endre

English

In order to test an alternative method on freshwater pollution with heavy metals, individuals of Unio crassus were exposed in cagcs in polluted reaches of the Some? River. Their time-related bioaccumulation was registered and compared with control samples

Sárkány-Kiss Endre

Braun Mihály

University of Szeged

T I S C 1 A m o n o g r a p h s c r i e s ( 2 0 0 2 ) . 6 . 71 K0 USING BIVALVES IN AN ALTERNATIVE TESTING METHOD OF FRESHWATER POLLUTION WITH HEAVY METALS Sárkány-Kiss Andrei and Braun Mihály Abstract In order to test an alternative method on freshwater pollution with heavy metals, individuals of Unio crassus were exposed in cagcs in polluted reaches of the Some? River. Their time-related bioaccumulation was registered and compared with control samples. Keywords: biomonitoring, bioaccumulation, pollution ecology Introduction The heavy metal content of the natural waters and of the sediments reflects the gcochcmical particularities of the examined place; their quantities usually do not cxceed the tolerable values for the organisms. Due to human influences, the concentration of heavy metals may increase in the water, as well as in the sediment. The aquatic organisms accumulate these elements along the trophic chain. Molluscs arc particularly well known as bioaccumulators of some of these metals (Fuller, 1974; Burky, 1983; Lakatos el al. 1990), taking them either directly from the environment, or indirectly through the food. The metals are stored in the soft parts of the body, as well as in the valves. It is known that the unionids molluscs arc great plankton consumers and it was experimentally demonstrated that unicellular algae arc able to retain during 14 days 78 - 98 % of the heavy metal contcnt (in positive relation with the concentration) of the culture media (Nagy-Tóth, F. and Adriana Barna, 1982). Due to these qualities of the unionid molluscs, many authors suggest their use as bioindicators in this sense and recommend in a particular way the use of gills as indicator organs for the heavy metal bioaccumulation (Salánki el al. 1991). Many studies demonstrate the sub-lethal and lethal toxic effects of these xcnobiotics in different organisms, the modification of their metabolism (Nagy-Tóth, 1981), as well as the appearance of certain malformations (Szító, 1994) or other modifications in the cells of different organs, or even the modifications of the nervous system (Serfőző, 1993). 93 The indicator value of the aquatic organisms is more obvious in the case of the temporary and punctiform pollutions, in which neither the water nor the sediments contain an excessive quantity of pollutant, but the organisms still bear the sign of modifications produced by them (Moore - Ramamoorthy, 1984, ap. Dévai, 1993). The Unionidae molluscs accumulate with a high intensity heavy metals, especially in gills. Because of their relative low mobility, these organisms are very good for testing the pollution degree of rivers with xenobiotics. The catastrophic accidental pollution with cyanides and heavy metals caused by the Aurul industrial unit, from Bozánta Mare, determined a drastic effect on the aquatic communities from the Some? and Lapu? rivers. Investigating the consequences during the years of 2000 - 2002 we noticed a serious load of heavy metals content in sediments. Afterwards the bioaccumulation was also researched, by using those organisms that are able to survive in lower reaches of the Some? River, namely fish and vascular plants. The latter showed high levels of heavy metals, but this could be due to sediments and biotecton settled on their organs, that are hard to be removed during the chemical analyses. Regarding the experiments with fish, in the year 2000 the metals' contents in the specimens captured in the exposed river sector were lower than those registered in individuals from the upper reaches. This proves that the fish that once lived in the polluted river sector died out, and were replaced after the environmental recovery, with other individuals belonging to different species, coming from upstream the river and its tributaries. These arc the reasons why wc decided to use bivalves as an alternative method of biomonitoring, but in the lower sector of the river this group is absent at present. Therefore individuals of Unio crassus Philipsson, 1788 had to be brought from the Lapu? River Defile and afterwards from the Some? River Defile from Ticáu, which still shelter abundant populations of naiads, and exposed in underwater cages in the Some? River. Materials and method In order to accomplish this experiment, on 18 October 2001 we collected manually 60 specimens from the Lapu? Defile (Ohaba de Padure sampling station). From these, 10 specimens were used as control samples (determining their heavy metal content), the other ones were exposed in the Some? River at Meri?or, downstream the discharge of the Lapu? River. These were held in plastic cages, and were removed for analyses in groups of 10 individuals after 7 days (on 25.10.2001), 21 days (on 08.11.2001) and 147 days (on 14.03.2002). After 08.11.2001 a very cold period followed and the Some? River froze, so we were unable to sample other individuals until the spring. The heavy metal content of muscles and gills were determined. 94 Results and discussion The results of the gills' analyses demonstrate a high bioaccumulation rate of copper and lead, in the first 7 days and, in a smaller degree, after 21 days. At this time the temperature of the water was higher, 7-8 °C. After this date, when the temperature of the water has fallen close to 0 °C, the mussels started to "hibernate" and catabolyze their own substances, so the heavy metal content decreased as well (Tab. 1, Fig. 1, 2) until the spring. T a b l e 1. H e a v y me ta l c o n t e n t of musse l s in 2001 Sample Cu Pb Mussels Sampling site Date mg/kg mg/kg Unio crassus/muscle -control Lâpuij - Defile 18.10.2001. 14 1 Unio crassus/gills- control Lapu$ - Defile 18.10.2001. 144 14 U. crassus/gills Some? - Meriçor 25.10.2001. 259 19 U. crassus/muscle Some? - Merisor 25.10.2001. 10 <1 U. crassus/gills Somes - Meriçor 08.11.2001. 171 27 U. crassus/muscle Somes - Merisor 08.11.2001. 5 <1 U. crassus/gills Somes - Merisor 14.03.2002. 77 13 U. crassus/muscle Somes - Merisor 14.03.2002. 8 <1 Somes at Merisor -14 Mar.2002 Somes at Merisor -8 Nov. Somes at Merisor -25 Oct. control - 18 Oct. 0 50 100 150 200 mg/kg dry tissue Fig. 1. T h e d y n a m i c s of heavy me ta l b i o a c c u m u l a t i o n in gills 250 300 • Pb • Cu 95 In a very sharp contrast, the analyses of muscles illustrates another pattern, namely that all samples, despite the period, showed lower contents of heavy metals than the control. Led is continuously decreasing, while the copper registered an increased value in spring 2002. compared to the fall of 2001. These peculiarities suggested that the gills arc much sensitive in heavy metals monitoring than muscles. This first experience proved that the method is applicable only in the warm seasons, during which the bivalves arc active. During the summer of 2002 the study was repeated in a similar manner but. finding out that the controls already have a ccrtain amount of heavy metals, bccausc of the residual mining waters discharged from the upper sectors, we used individuals from the Jicau Defile, the Some? reach upstream the confluence with the Lapu? tributary. The results are not available yet, but during the summer in only 9 weeks 60 % of these bivalves died, and the others were seriously weakened, having a pale colour of the tissues, as compared lo the control samples. Is worth mentioning that after the first 7 days the Sr content of the gills has increased. According to Cootc & Trompcttcr (1995) one of the possible reasons of this increase should be the effect of stress. Somes al Merisor -14 Mar.2002 Somes at Merisor -8 Nov. Somes at Merisor -25 Oct. control - 18 Oct. • Pb • Cu 0 50 100 150 200 mg/kg dry tissue Fig. 2. T h e d y n a m i c of heavy me ta l h i o a c c u m u l a t i o n in musc le 250 300 96 Acknowledgement The study was supported by the Inco Copcrnicus Projcct ICA2-1999-10065. References Barna, A., Nagy-Tóth, F. (1993) - Anal ize ficofiziologicc in zona Zlatnei. (Pshycophysiological analyses in the Zlatna zone) - St. ccrc. biol., Seria biol. veget., 45, 1, 107 - 117. Burky, A.J. (1983) - Physiological Ecology of Freshwater Bivalves. The Mollusca, Ecology, cds. W.D. Russcl-Huntcr, Academic Press, Inc., Orlando, San Diego, San Francisco, New York, London, Toronto, Montreal, Sydney, Tokyo, Sao Paulo, 6, 281 -330. Coote, G.E. and W. J. Trompctter (1995): Proton microprobe studies of fluorine distribution in mollusc shells. 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(1993): Necrotic effects of the xcnobiotics accumulation in the central nervous system of a crayfish (Astacus leptodactylus Eschz.). - Acta biol. Szeged. 39, 23 - 38. Szító, A. (1994) - Monitoring in aquaculture. - First Dutch - Hungarian Course on Biomonitoring in Hungarian Waters: Application an Training, Göd. Hungary. Wachs, B. (1985): Bioindicators for the heavy metal load of river ecosystcms. - in Heavy metals in water organisms (Ed. Salánki, J.). Akad. Kiadó, Budapest. 179 - 190. ANDREI SÁRKÁNY-KISS "Babc$-Bolyai" University. Faculty of Biology and Geography, Dept. of Ecology and Genetics, 5-7 Clinicilor St., RO 3400 Cluj-Napoca, asarkany@hasdeu.ubbcluj.ro; BRAUN MIHÁLY University of Debrecen Department of Inorganic and Analytical Chemistry H-4010 Dcbrcccn P.O.Box 21, Hungary braun@tigris.klte.hu 98 

Using bivalves in an alternative testing method of freshwater pollution with heavy metals

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Using bivalves in an alternative testing method of freshwater pollution with heavy metals

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