Objective: Bioactive glass 45S5 is a surface reactive glass-ceramic biomaterial, developed in 1969.
BAG 45S5 with particle size of 20-60 nm has the ability of bone regeneration, broad spectrum
antibacterial effect, repairs and replaces diseased or damaged bone. The aim of this study was to
evaluate the antibacterial activity and determine MIC and MBC values of nano-BAG45S5 on
Streptococcus mutans.
Methods: In this study the in vitro Antibacterial activity of polycrystalline and glass forms of nanoBAG
45S5 was evaluated. Bacterial susceptibility to test materials was examined by antibiogram
test. Afterwards MIC and MBC assays were conducted via broth dilution, disc diffusion and colony
count methods.
Results: Despite amorphous nano-BAG 45S5, poly-crystalline form had antibiogram negative test
result. In broth dilution test, the optical absorbance of test dilution of 50mcg/ml and higher
concentrations were equal to negative control’s optical absorbance and their inhibitory zone diameter
were measured 10.0mm in disc diffusion test. No colony was observed on the culture media of test
dilution of 200mcg/ml and higher concentrations.
Conclusion: Streptococcus mutans (ATCC 35668) is not susceptible to poly-crystalline nanoBAG45S5.
Amorphous nano-BAG45S5 is bacteriostatic against Streptococcus mutans. MIC and
MBC values for amorphous nano-BAG45S5 were 50 ppm and 200 ppm, respectively