Fortunato, Gianuario

Manaia, Célia

Nunes, Olga

Vaz-Moreira, Ivone

English

Repositório Institucional da Universidade Católica Portuguesa

7-11 July 2019 | Glasgow, Scotland | www.fems2019.org8th Congress of European MicrobiologistsABSTRACT BOOKIn collaboration with1279  PW145 Fate of Pseudomonas aeruginosa and blaVIM in soil under selective pressure by copper and zinc  Gianuario Fortunato1, Ivone Vaz-Moreira1, Olga Nunes2, Célia Manaia1  1Universidade Católica Portuguesa, Centro de Biotecnologia e Química Fina , Porto, Portugal 2Universidade do Porto, LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Porto, Portugal   Background: Besides the natural resistance to antimicrobials present in soil, antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) can be discharged in soil through irrigation or other agricultural practices, which can also be a source of other contaminants such as metals. It is hypothesized that metals may generate selective pressure enhancing the survival of bacteria and/or of their ARGs. Objectives: This study aims to assess the survival of a multidrug-resistant Pseudomonas aeruginosa strain in soil contaminated with metals as well as the fate of the blaVIM gene. Methods: Assays were performed in sandy soil microcosms inoculated with P. aeruginosa strain H1FC49, in the presence and absence of potential selective pressures, generated by 20 mM CuSO4 and ZnSO4 or by Cu(NO3)2 and Zn(NO3)2. Microcosms were incubated at 25°C for 14 days and monitored weekly through the enumeration of P. aeruginosa on Cetrimide Agar + Nalidixic Acid and quantitative PCR of the housekeeping gene ecf and the acquired genes blaVIM and intI1. Results: The survival of P. aeruginosa in soil amended with copper and zinc nitrate was significantly lower than in a non-amended soil, while no significant difference was observed in presence of sulfate salts. After 14 days of incubation, in presence of the metal selective pressure, the P. aeruginosa housekeeping gene ecf decreased more than the acquired ARG blaVIM. These results suggest that antibiotic resistance selection may occur mainly at the molecular rather than at the cellular level. The molecular mechanisms behind these variations will be further discussed.      

Fate of pseudomonas aeruginosa and blaVIM in soil under selective pressure by copper and zinc

https://repositorio.ucp.pt/bitstream/10400.14/29783/1/PW145%20Fate%20of%20Pseudomonas%20aeruginosa%20and%20blaVIM%20in%20soil%20under%20selective%20pressure%20by%20copper%20and%20zinc.pdf

Fate of pseudomonas aeruginosa and blaVIM in soil under selective pressure by copper and zinc

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Repositório Institucional da Universidade Católica Portuguesa