Castro, Paula M.L.

Gonçalves, David

Mascolo, Giuseppe

Moreira, Irina S.

Murgolo, Sapia

English

Repositório Institucional da Universidade Católica Portuguesa

                                                      2ndInternational Meeting on New Strategies in Bioremediation Processes PORTO | 24-25th October 2019 BOOK OF ABSTRACTS Oral Communications  BioRemid2019  16 Strain Rhodococcus sp. ED55 isolated from a WWTP in Macao degrades β-estradiol and removes toxicity of treated effluents OC 03 Irina S. Moreira 1, Sapia Murgolo 2, David Gonçalves 3, Giuseppe Mascolo 2, Paula M.L. Castro 1  1 Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal 2 CNR, Istituto di Ricerca Sulle Acque, Via F. De Blasio 5, 70132 Bari, Italy 3 Institute of Science and Environment, University of Saint Joseph, Macau SAR, China ismoreira@porto.ucp.pt   Key words: β-estradiol, biodegradation, Rhodococcus sp. ED55, wastewater, toxicity.  Abstract β-estradiol (E2) is an endogenous steroid hormone excreted by humans and animals. Due to incomplete removal on wastewater treatment plants (WWTP) and animal waste disposal, this micropollutant reaches the environmental compartments, being detected in WWTP effluents, surface water, soil and sediments [1]. This is an issue of great concern due to its endocrine disruption potential. The main objective of the present study was to evaluate the biodegradation of E2 by a bacterial strain – Rhodococcus sp. ED55 - isolated from the sediments of a discharge point of a WWTP in Coloane, Macau. Biodegradation experiments were performed in synthetic mineral medium and in wastewater from a municipal WWTP (Parada, Maia – Portugal). Strain ED55 was able to completely degrade the supplied amount of E2 in few hours, both in synthetic medium and in municipal wastewater. Estrone (E1) was identified as intermediate degradation metabolite, by comparison with a commercial standard. The detection and identification of other biodegradation intermediates by UPLC-QTOF/MS/MS is ongoing, aiming at elucidation of the metabolic pathway of degradation. Moreover, the bioaugmentation with E2 significantly improved the natural attenuation of the compound in municipal wastewater. The acute test with luminescent marine bacterium Vibrio fischeri revealed elimination of the toxicity of the treated effluent. Bacterial strain Rhodococcus sp. ED55 can potentially be applied for bioaugmentation of bioreatores for the enhancement of wastewater treatment.   Acknowledgements This work was supported by National Funds from FCT - Fundação para a Ciência e a Tecnologia - through the project AGeNT - PTDC/BTA-BTA/31264/2017 (POCI-01-0145-FEDER-031264) and project 011/2014/A1 funded by FDCT - Macao Science and Technology Development Fund. We would also like to thank the scientific collaboration of CBQF under the FCT project UID/Multi/50016/2019.  Bibliography [1] Zhang, C., Li, Y., Wang, C., Niu, L., Cai, W. (2016). Crit Rev Environ Sci Technol 46, 1-59.     

Strain rhodococcus sp. ED55 isolated from a WWTP in Macao degrades β-estradiol and removes toxicity of treated effluents

https://repositorio.ucp.pt/bitstream/10400.14/29660/1/Strain%20Rhodococcus%20sp.%20ED55%20isolated%20from%20a%20WWTP%20in%20Macao%20degrades%20%c3%9f-estradiol%20and%20removes%20toxicity%20of%20treated%20effluents.pdf

Strain rhodococcus sp. ED55 isolated from a WWTP in Macao degrades β-estradiol and removes toxicity of treated effluents

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Repositório Institucional da Universidade Católica Portuguesa