Previous studies have found that peripherally inserted central catheter (PICC) is effective for delivering an intravenous therapy. However, few studies were found to compare the effectiveness of PICC with peripheral intravenous access. The purpose of this study was to compare the effectiveness of intravenous therapy using peripheral intravenous access and PICC in hospitalized neonates. This study was a descriptive comparative. By using a purposive sampling technique, 32 neonates were involved as subjects of peripheral IV access group (n=16) and PICC group (n=16). Data were collected using observation forms for 24 hours within 5 days in a row. A Fisher Exact test was utilized to analyze the data. The results indicated that PICC was more effective than peripheral intravenous access (Ο=0.00) in providing intravenous therapy for neonates. Accordingly, PICC is recommended for neonates requiring intravenous therapy in the hospital