Neisseria gonorrhoeae 유래 재조합 탄산무수화 효소의 특성 연구

Abstract

MasterSince the industrial revolution, concentration of carbon dioxide (CO2), known as a main greenhouse gas, in the air has been increasing. As the global warming start to be a global problem, many researchers have been focusing on reducing CO2 concentration. Converting CO2 in biological way is considered as one of most promising way in many respects including using solar energy as its fundamental energy source. Enzyme carbonic anhydrases work as key roles in CO2 in biological way. Previously, recombinant Neisseria gonorrhoeae carbonic anhydrase (ngCA) was produced successfully. In this research, recombinant ngCA was produced in larger scale and investigated its activity in various conditions for potential applications in industrial situation. In larger scale, recombinant ngCA was able to be produced with better yield (~3 g soluble ngCA per l liter culture) and subsequently, the cost for ngCA production was significantly decreased. For the characterization of recombinant ngCA, the activity of enzyme after incubation in different environments closer to actual industrial process was checked, with different metal ions (Ca2+, Fe2+, Ni2+), different anions (HS-, CN-), and in different temperatures (4°C, 50°C). As a result, the activity was affected by incubation with different ions but it decreased by the concentration decreased. The activity was also decreased by incubation at 50°C but the effect by the ion showed the similar tendency as in 4°C