Shoot (A-C) and root (D-F) ion content for HKT1;1 native overexpression lines. Statistical significance was determined using Tukey’s HSD test between each line within treatments. Bars with the same letters indicate no significant difference (p < 0.05). Error bars represent standard error of the mean where n = 12–18 plants.</p

Aaron J. Lorenz (359497)

Aaron J. Schmitz (711662)

Anne-Aliénor Véry (4067899)

Chi Zhang (9857)

Fouad Razzaq A. Al Shiblawi (4067896)

Harkamal Walia (69555)

Kan Liu (250462)

Malachy T. Campbell (711660)

Nonoy Bandillo (4067893)

Qian Du (280345)

Sandeep Sharma (193218)

PLoS Genetics

Swedish

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Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content - Fig 7

Johansson, Ingrid,

Göteborgs universitets publikationer - e-publicering och e-arkiv

Arbetsplatser i Kortedala och Gårdsten : branschstruktur och lokaliseringsmönster i två bostadsområden i Göteborg /

Salinity is a major factor limiting crop productivity. Rice (Oryza sativa), a staple crop for the majority of the world, is highly sensitive to salinity stress. To discover novel sources of genetic variation for salt tolerance-related traits in rice, we screened 390 diverse accessions under 14 days of moderate (9 dS·m-1) salinity. In this study, shoot growth responses to moderate levels of salinity were independent of tissue Na+ content. A significant difference in root Na+ content was observed between the major subpopulations of rice, with indica accessions displaying higher root Na+ and japonica accessions exhibiting lower root Na+ content. The genetic basis of the observed variation in phenotypes was elucidated through genome-wide association (GWA). The strongest associations were identified for root Na+:K+ ratio and root Na+ content in a region spanning ~575 Kb on chromosome 4, named Root Na+ Content 4 (RNC4). Two Na+ transporters, HKT1;1 and HKT1;4 were identified as candidates for RNC4. Reduced expression of both HKT1;1 and HKT1;4 through RNA interference indicated that HKT1;1 regulates shoot and root Na+ content, and is likely the causal gene underlying RNC4. Three non-synonymous mutations within HKT1;1 were present at higher frequency in the indica subpopulation. When expressed in Xenopus oocytes the indica-predominant isoform exhibited higher inward (negative) currents and a less negative voltage threshold of inward rectifying current activation compared to the japonica-predominant isoform. The introduction of a 4.5kb fragment containing the HKT1;1 promoter and CDS from an indica variety into a japonica background, resulted in a phenotype similar to the indica subpopulation, with higher root Na+ and Na+:K+. This study provides evidence that HKT1;1 regulates root Na+ content, and underlies the divergence in root Na+ content between the two major subspecies in rice

Campbell, Malachy T

Bandillo, Nonoy

Al-Shiblawi, Fouad

Sharma, Sandeep

Liu, Kan

Du, Qian

Schmitz, Aaron J

Zhang, Chi

Véry, Anne-Aliénor

Lorenz, Aaron J

Walia, Harkamal

HAL Descartes

Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content.

Malachy T. Campbell

Nonoy Bandillo

Fouad Razzaq A. Al Shiblawi

Sandeep Sharma

Kan Liu

Qian Du

Aaron J. Schmitz

Chi Zhang

Anne-Aliénor Véry

Aaron J. Lorenz

Harkamal Walia

Crossref

Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content

HAL: Hyper Article en Ligne

ProdInra

Archive Ouverte en Sciences de l'Information et de la Communication

Malachy T Campbell

Fouad Razzaq A Al Shiblawi

Aaron J Schmitz

Aaron J Lorenz

Directory of Open Access Journals

Root (A-C) and shoot (D-F) ion content of HKT1;1 and HKT1;4 RNAi plants. Asterisks indicate significance as determined using Tukey’s HSD: ***: p < 0.001; *: p < 0.05. Pairwise comparisons were made between lines within each treatment. Error bars represent standard error of the mean where n = 12–20 plants.</p

Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content - Fig 5

<div>Salinity is a major factor limiting crop productivity. Rice (Oryza sativa), a staple crop for the majority of the world, is highly sensitive to salinity stress. To discover novel sources of genetic variation for salt tolerance-related traits in rice, we screened 390 diverse accessions under 14 days of moderate (9 dS·m-1) salinity. In this study, shoot growth responses to moderate levels of salinity were independent of tissue Na+ content. A significant difference in root Na+ content was observed between the major subpopulations of rice, with indica accessions displaying higher root Na+ and japonica accessions exhibiting lower root Na+ content. The genetic basis of the observed variation in phenotypes was elucidated through genome-wide association (GWA). The strongest associations were identified for root Na+:K+ ratio and root Na+ content in a region spanning ~575 Kb on chromosome 4, named Root Na+ Content 4 (RNC4). Two Na+ transporters, HKT1;1 and HKT1;4 were identified as candidates for RNC4. Reduced expression of both HKT1;1 and HKT1;4 through RNA interference indicated that HKT1;1 regulates shoot and root Na+ content, and is likely the causal gene underlying RNC4. Three non-synonymous mutations within HKT1;1 were present at higher frequency in the indica subpopulation. When expressed in Xenopus oocytes the indica-predominant isoform exhibited higher inward (negative) currents and a less negative voltage threshold of inward rectifying current activation compared to the japonica-predominant isoform. The introduction of a 4.5kb fragment containing the HKT1;1 promoter and CDS from an indica variety into a japonica background, resulted in a phenotype similar to the indica subpopulation, with higher root Na+ and Na+:K+. This study provides evidence that HKT1;1 regulates root Na+ content, and underlies the divergence in root Na+ content between the two major subspecies in rice.</div

Allelic variants of OsHKT1;1 underlie the divergence between indica and japonica subspecies of rice (Oryza sativa) for root sodium content

Arbetsplatser i Kortedala och Gårdsten : branschstruktur och lokaliseringsmönster i två bostadsområden i Göteborg /

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Göteborgs universitets publikationer - e-publicering och e-arkiv

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HAL: Hyper Article en Ligne

ProdInra

Archive Ouverte en Sciences de l'Information et de la Communication

Directory of Open Access Journals

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