Soil bacterial isolants from harsh environment

Bollen, W. B.

Kemper, K. M. B.

English

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6FINAL REPORT
W eroon,,anti sm ';tudy : Bacterlul 7solants
from Haruki Iar,vironmcnts
Jc t Propul.i i on T aborato ry
C-lifornia Inst9. Wtc of Te chnoJ oLV
Contract 95U'(q3
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Thi% work was "formed for the Jet hrnpnl%i,)n l.ahoratory,
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National Acrotiamia tuid Sp iw Administratian under
Contract NAS7-lcw.
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(ti^ar^r:orly /ifiE31fi^ ^.:^t in I.icrJ'r-Iolov)` 
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Corvallis, Orc f ocn
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FITUIL REPORT
Part 1
ok
Microori anicm Study : Bacterial I"solant:s
f'ro^) Parish Environments
Jet Propulsion Laboratory CIT Contract 950783
Professor 67. R. Bollen
Department of Mlicrobiology
Oregon State University
Corvallis, Oregon
Februnry 20, 1970
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FRMEDING PAGE BLANK NOT FILMED.
Fina l Report
Pr rt 1
This report is a summary of isolants rc.cvived fr om
1 rota Dr. koy F'. Came rot i, Senior Scienti.sl , Hor,ciencc- Section,
.lct Propulsion Laboratory, and of thc• isolants identified.
Procedure;, methods and ►nediu empl oyed lcive been described
in the series of Progress Reports listed in the t. ► ble presented
herewith, Generic Find species na mes wore applied whert. po^sible.
Isolants not narned were unidentified for one of Hie following
reasons:
1) 'they failed to grow on sub:;eauent transfer. This is
characteristic of mai)y sui.l isoiant.s; presum^ibly some
nutritional factor in the soil or provided by -:ome
^^ssociated microbe is not carried over by the inoculum
in subsequent cultures.
2) The isolant grew in subsequent cultures on the various ter
media but the results did not conf' ,3rm to any specie.-
dc seription in Be.rgey's Manual or in current literature.
1j ► such cases only a generic or a morphological designation
was given.
All except prel iHnary and stunmary reports were accompnni.ed
by Descriptive Systematic Charts with photographs.
At termination of the contract or. March 1, 1969 funds remai.ni..ng
were sufficient to continue the work on a reduced basis until
June 30, 1969. Since this later date only the last lot of isolants
shipped to us by Dr. Cameron remain to be processed. Thee are now
being worked on by Mrs. Karen Kemper, 3317 Somerset Drive, Castro
Valley, California 94546 (Telephone: 415-538-4502), who will complete
the study and ident ifications as time allows,  Compl etion of this
work is essential for her MS thesis, which is on "Soil Dipht-hero.Ws
from llarsh l:nviron ►vents. " Many of the cultures submitted by Dr. Cameron
proved to be diphtheroids, about .ahi(:h little is knowii or publ.ishc,.t.
It is expected that tl^e thesis will prove to be a valuable contribution
to knowledge of this group of soil. bacteria.
Wlien available, results for the last lot of isolants will be
submitted to Dr. Cameron as a Supplementary Report.
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tT'1 NAL REP IRT
Part 2
final
Microorganism Study, CIT Contract No. 950783
Systematic Dosc ript i.on of Antarctic Soil rso lant's
Professor W. B. pollen, Microbiologist
and
Karen M. Kemper
(Formerly Assistant in Microbiology)
Oregon State University, Corvallis, Oregon
May 22, 1970
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This report supplcincnts omd conch0c.-, my T'in:al Report of
February 20, 1970. It is based upon careful exr ► mination :mJ con-
8ideration oI' the Desvri1)tive charts and dat::a iu t-hc notebooks
obtained from Mrs. Karen M. K empc r and sent to inc by Dr. koy 1.. Came rora ,
April 13, 1970, for rappraisal. Although the work h: :5 not attained the
extant of completion :ant icipated, the progress hat; beml substantial ,
considering the grant number of cultures involved and the conditions
under which Mrs. Kemper attempted to complete the task.
Following, according; to sources of irsolant-s, are ( I) I i.sts of
identifications, and ()) lists of incompletely studied isolants.
List of 73 Antarctic sail isolants, with iderat if - icrations where possihle l/
Asvard	 ltan iT es	 Isolants
501 Unidentified rod
661 Ar.throbacter sp.
665 Microc occus sp.
667 Unidentified rod
672 Art-hrobacter or pleomorphic coccus
673 Unidentified coccus
675 Unidentified rod
676 Unidentified rod
K is&David
636al Actinomycete + yeast
637.01 Actinomycete
644al. Actinomycete
Marble Point
562 Actinomycete
McKelvev Valley Isol.arats
500 Mycobacterium sp.
500A Diphtheroid
50OA-1 Mycoccus
50OA-2 Arthrobacter sp.
500R' bacillus sp.
501A A rthrobacte a: sp.
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501A-1 Un ident i f ied rod
501C Diphtheroid
502 Mycoccus 81).
502A. Mveobacter- ium sp.
50213. Bacillus cert us
502-1 1)i.pl.i lie roid
502 -2 Unidentified coccus
503 Mycoccus sp.
503-1 Unidentified roc)
503-2 Arthrobacter sp.
503-3 Arthrob;tctcr sp.
503-A 1)i phthero id
503A-1 Pleomorphi.c rod
503A-2 Paci] ]us	 pumilu.',
50311 Bacill us
	cereus
507A Mi croccus urc ac
507Ai Flagellatcd rod
508A Sa rcina sp.
5088 Diphtheroid
510A-1 P. wil l lls congulans
j0A-2 Bacillus
	 cc r"cus
51013-1 Racil ius cereus
510I3-2 Bac 11 l us
	 ce rous
511A Unidentified coccus
513A Diphtheroid
514A-1 Diphtheroid
51413 Failed to grow
515A Bacillus megaterium
Victoria Vallev Isclants
537A Mycoccus ruber
53713 Bacillus cereus
538A B3Icillus	 cereus
539A Diphtheroid
539aA, Bacillus	 circul-ans
5398 Diphtheroid
539aB Micrococcus al))us albidus
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540A
	
v.:ci11u5 cervus
542A	 Uiplitheroid
54 3B
	
Pac i 1 l us cercus
575A1.	 Yeast.
575Ac	 Ycnst,
Wheeler Valley 1. o1- ► nts
609 1_cti1101nycc-te i yeast
609-1 Un ident- if iv (I n ► ic rococcut;
609-2 Unidentified iniciococcu:,
609-3 Utt ident i f f ec: rod
609-4 Unidentified pleomorphic rod
611 Act ittomyce.te
611-1 Arthrobacter sp.
611-2 Unidentified rod
614 Actinomycete + yeast
617 Actinomycete
618 Actinomycete
619 Actinomycete + yeast.
620 Actinomycete
1/ Reasons for lack of system^itic identification are:
(1) failure. to grow on subsequent transfer;
(2) results of growth on various test media do not conform to any
published description or
(3) physiological and/or biochemical tests not completed.
The foregoing, lists include the following;:
7 Arthrobacter 1.07.
15 Bacillus 217.
10 Diphtheroids 1417.
3 Microccus 4"/.
2 Mycobacterium 37.
4 Mycoccus 6%
1 Sarcina 17,
11 Actinomycete 157,
2 Yeasts 30,1.
5 Unidentifiable cocci 77.
12 Unidentifiable rods 16'-
1 failed to grow
1
•
1
As noted in Lite h.nul Ruport o f 1 cimiary 70 specii-s^ (fit ll,^ccii 11 us
diphtheroids rind Streptowycec predominated among; the isolants.
Arthrobnuter, elovely rel; ► ted to the diphtheroids, n1so were rc c l-
atively abundant. As previously observed these forms c, eem most
adaptable to harsh er ► virronwent.s, regurdle:.s of climatic: tcuip ► rat:ure.
NOTE: Microccus should be indicated as Micrococcus.
	
Aicoccus should
be indicated wi 14+ cococcuc.
•lA
On the following 92 iuol.t"tF only the "Itu"'I characters - colony,
slope and broth culture descriptimnc - were made. 'This information is
not sufficient for Identif ical ion.
Asga rd 11nitEe
503 9 514, 615, 663 0 664, 666 9 666 9 670 9 675 0 676
Beacon yullcy
533
King David (King Valley (David Valley)
636-1, -2; 637-1, -2; 638-1 9 -?, -3 9 -4, -5;
639-1 1 -2 9 -3. -4 9 -5; 640-1 ) -2, -3; 642;
643-1, -2 1 -3; 644
Marble Point
562; 562-1; 562-3
M^Ke1veY
503 2 506-1 9 -2 9 -3; 509, 510, di ll, 513 9 514
01y► npi c Range
651-1 1 -2; 652
Victoria VAley
538; 539x; 539-1, -2; 574; 575-1, -2, -3; 576
Wheeler V;.' ley
609; 611-1, -2, -3; 612; 613-1, -2, -3 1 -4 1 -5 1 -6 1 -7, -8, -9, -10;
514-1 1 -2, -3 2 -h, -5; 615-1, -2, -3, -4, -5 9 -6; 616; 617;
618-1, -2; 62.0-1, --2; 622-1, -2, -3


Microorganism study - Bacterial isolants from harsh environments Final report

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