: Phlegm is cough showed an infection and inflammation of the respiratory tract. The purpose of this study is to identify aerobic bacteria in patients with cough with phlegm in Interna Polyclinic Prof. dr. R. D. Kandou. This is a descriptive study with 30 samples of patients with phlegm cough was obtained. Thirty sputum were collected for microbiological testing by standard culture techniques. The result of this study is 30 patients with phlegm cough is men (20; 66,7%), woman (10;33,3%), aged 21-35 years old (4; 13,3%), 36-50 years old(8; 26,7%), 51-65 years old (10; 33,3%) dan ≥66 years old (8; 26,7%). Thirty samples of Sputum examines, 29 sample showed growth of microba and got two bacteria positive gram, there are Streptococcus (7; 24,1%) and Staphylococcus aureus (1;3,4%); and eight bacteria negative gram, there are Proteus, (6; 20,7%), Enterobacter aerogens (5;17,2%), Klebsiella ozaenae, Acinetobacter baumanii (2;6,9%) dan Citrobacter difersus, Escherichia coli, Hafnia alfei, Seratia marcescens (1; 3,4 %). In this study we found that the most bacterial positive gram causes phlegm cough is Streptococcus, and negative gram are Proteus and Enterobacter aerogenes