With the rapid development of tissue engineering and gene therapy, collagen-based biomaterials are frequently used as cell transplant devices; an example is bio-artificial skin substitutes [1-3]. In this study, we determined the effects of two different cell-seeding methods, monolayer and suspension seeding, on the rate of contraction of free-floating collagen and collagenglycosaminoglycan (GAG) gel matrices in vitro. 3T3 mouse fibroblasts were cultured in/on the matrices for up to 7 days
