Hiroaki Azukizawa

Ichiro Katayama

Kenichi Kato

Clinical and Translational Allergy

PubMed

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Analysis of B cell subsets in severe cutaneous adverse reaction

POSTER PRESENTATION Open Access
Analysis of B cell subsets in severe cutaneous
adverse reaction
Hiroaki Azukizawa1*, Kenichi Kato2, Ichiro Katayama2
From 6th Drug Hypersensitivity Meeting (DHM 6)
Bern, Switzerland. 9-12 April 2014
Background
T cells play an important role in the pathogenesis of severe
cutaneous adverse reaction (SCAR), while the role of B cell
immunity in SCAR is unclear. It has been reported that
number of B cell and serum IgG are decreased in the
patient’s peripheral blood of drug-induced hypersensitivity
syndrome (DIHS) / drug reaction with eosinophilia and
systemic symptoms (DRESS) in the acute stage. Regulatory
B cell (Breg) is a IL-10 producing cell and negatively regu-
lates cellular immunity in the mouse autoimmune disease
model. Also in the mouse model, B-1 B cell secretes
immunoglobulin against microbial infection in a T cell-
independent manner. Recently, Breg and B-1 B cell were
identified as minor populations of human B cells in the
peripheral blood, however, the roles of these B cells in
SCAR are unknown. Here, we studied the ratio of Breg
and B-1 B cells in the peripheral blood B cells of SCAR
patients.
Method
Three patients of toxic epidermal necrolysis (TEN), DIHS/
DRESS, and three healthy controls were involved in this
study. Peripheral blood mononuclear cells of SCAR
patients in both acute stage and recovery stage were
stained. CD24hi, CD27+, CD19+ cells and CD27+, CD43
+, CD20+ cells were analyzed as Breg and B-1 B cells,
respectively.
Results
The ratio of B cells in the peripheral blood was decreased
in the acute stage SCAR. Furthermore, the ratio of Breg in
the CD19+ cells was decreased in the acute stage of DIHS.
On the other hand, the ratio of B-1 B cells was different
among patients of TEN and DIHS and there was no clear
tendency.
Conclusion
Although the pathological roles of Breg and B-1 B cell in
SCAR are still unclear, they might contribute to drug-
specific T cell activation or herpes virus reactivation.
Authors’ details
1Department of Dermatology, Course of Integrated Medicine, Osaka
University, Japan. 2Osaka University, Department of Dermatology, Japan.
Published: 18 July 2014
doi:10.1186/2045-7022-4-S3-P44
Cite this article as: Azukizawa et al.: Analysis of B cell subsets in severe
cutaneous adverse reaction. Clinical and Translational Allergy 2014
4(Suppl 3):P44.
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1Department of Dermatology, Course of Integrated Medicine, Osaka
University, Japan
Full list of author information is available at the end of the article
Azukizawa et al. Clinical and Translational Allergy 2014, 4(Suppl 3):P44
http://www.ctajournal.com/content/4/S3/P44
© 2014 Azukizawa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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Analysis of B cell subsets in severe cutaneous adverse reaction

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