B Sumangala

K Anuradha

S Umamaheshwari

Sumana M Neelambike

BMC Infectious Diseases

PubMed

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POSTER PRESENTATION Open Access
Diagnosis of mycobacterial drug resistance in HIV
reactive patients by phenotypic and genotypic
assay - a comparative study
S Umamaheshwari1, Sumana M Neelambike1*, K Anuradha2, B Sumangala3
From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)
Chennai, India. 30 January - 1 February 2014
Background
Emergence of drug resistance has complicated the treat-
ment of Tuberculosis (TB). WHO reports India as one
among 27 “high burden” multidrug resistant (MDR) TB
countries. The study was carried out to detect drug
resistance of mycobacterial isolates in HIV patients.
Methods
The clinical specimens collected from HIV seropositive
AFB smear negative patients (Group A) with features of
TB, AFB smear positive, HIV reactive (Group B) and AFB
smear positive, HIV negative (Group C) were processed as
per standard protocol. They were inoculated onto Lowen-
stein Jensen media and the isolates obtained were sub-
jected to drug susceptibility test (DST) by proportion
method and Genotype MTBDR plus assay.
Results
In Group A out of 162 patients, 443 clinical samples were
collected and 76 mycobacterial strains were obtained from
67 (41%) patients. In this 69 (91%) were Mycobacterium
tuberculosis complex and 7 (9%) were Mycobacterium
avium complex. As per phenotypic method, of 76 isolates,
50 (65.8%) were sensitive to all drugs and 26 (34.2%) resis-
tant to one or more drugs. The MDR and resistance to at
least single drug of Group A, B and C were [5 (6.6%),
2 (6.7%), 4 (8%)] and [26 (34.2%, 3(10%), 8 (16%)] respec-
tively. MTBDR assay showed similar results except one
discrepancy i.e., one isolate was resistant to rifampicin that
was sensitive in proportion method.
Conclusion
The increase of MDRTB in high TB burden settings
stresses the need for DST. This helps in providing proper
treatment regimens, improve prognosis and prevent the
spread of drug resistant TB.
Authors’ details
1Department of Microbiology, JSS Medical College, Mysore, Karnataka, India.
2Department of Microbiology, Mysore Medical College and Research Centre,
Mysore, Karnataka India. 3Department of Microbiology, Mandya Institute of
Medical Sciences, Mandya, Karnataka, India.
Published: 27 May 2014
doi:10.1186/1471-2334-14-S3-P58
Cite this article as: Umamaheshwari et al.: Diagnosis of mycobacterial
drug resistance in HIV reactive patients by phenotypic and genotypic
assay - a comparative study. BMC Infectious Diseases 2014 14(Suppl 3):P58.
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* Correspondence: mnsumana12@gmail.com
1Department of Microbiology, JSS Medical College, Mysore, Karnataka, India
Full list of author information is available at the end of the article
Umamaheshwari et al. BMC Infectious Diseases 2014, 14(Suppl 3):P58
http://www.biomedcentral.com/1471-2334/14/S3/P58
© 2014 Umamaheshwari et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver
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Diagnosis of mycobacterial drug resistance in HIV reactive patients by phenotypic and genotypic assay - a comparative study

http://dx.doi.org/10.1186/1471-2334-14-S3-P58

Diagnosis of mycobacterial drug resistance in HIV reactive patients by phenotypic and genotypic assay - a comparative study

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