Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Flow rate and wall shear stress are important parameters for perfused cell culture systems and should be monitored. An easy and non-invasive method is the particle image velocimetry (PIV). In this work PIV was used to characterize a cell culture system with included peristaltic pump. The time-dependent flow profile was measured on several points of the chip for different pumping speeds to figure out which forces are applied to dissolved and adherent cells. The results can be used to improve the developed pump in respect to its layout, the excitation and the position within the chip