In the global osmoshock translational response in yeast, some gene products were translationally mobilized without transcriptional up-regulation. Conversely, other transcriptionally up-regulated mRNAs were translationally inhibited. Analogous changes occurred on the protein level. These translational responses were strongly dependent on Hog1 and Rck2

Albertsen M.

Alepuz P. M.

Asp E.

Bilsland E.

Bilsland-Marchesan E.

Brockmann R.

Buchan J. R.

Charles Boone

Cheng T. H.

Dahlkvist A.

de Nadal E.

Dunand-Sauthier I.

Fernandez-Ricaud L.

Francesc Posas

Gauss R.

Ghaemmaghami S.

Giaever G.

Gygi S. P.

Halbeisen R. E.

Hogan D. J.

Hohmann S.

Hounsa C. G.

Ingolia N. T.

Jonas Warringer

Krantz M.

Kuhn K. M.

Laloo B.

Lawless C.

Lu P.

Lü X.

Maeda T.

Malin Hult

Melamed D.

Melcher M. L.

Molin C.

Per Sunnerhagen

Posas F.

Powley I. R.

Preiss T.

Proft M.

Rajasekhar V. K.

Rep M.

Romero-Santacreu L.

Segal E.

Sergi Regot

Shenton D.

Smirnova J. B.

Stelling J.

Teige M.

Teixeira D.

Thorsen M.

Tomitori H.

Uesono Y.

Van Wuytswinkel O.

Washburn M. P.

Westfall P. J.

Wolin S. L.

Yale J.

Yang X. X.

Özcan S.

Molecular Biology of the Cell

English

PubMed

Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins including hexose transporters were enriched. Similarly, numerous mRNAs encoding cytoplasmic ribosomal proteins run counter to the overall trend of down-regulation and are instead translationally mobilized late in the response. Surprisingly, certain transcriptionally induced mRNAs were excluded from ribosomal association after shock. Importantly, we verify, using constructs with intact 5' and 3' untranslated regions, that the observed changes in polysomal mRNA are reflected in protein levels, including cases with only translational up-regulation. Interestingly, the translational regulation of the most highly osmostress-regulated mRNAs was more strongly dependent on the stress-activated protein kinases Hog1 and Rck2 than the transcriptional regulation. Our results show the importance of translational control for fine tuning of the adaptive responses

Warringer, Jonas,

Hult, Malin,

Regot, Sergi

Posas, Francesc

Sunnerhagen, Per,

Swepub

The HOG pathway dictates the short-term translational response after hyperosmotic shock [Elektronisk resurs]

Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins including hexose transporters were enriched. Similarly, numerous mRNAs encoding cytoplasmic ribosomal proteins run counter to the overall trend of down-regulation and are instead translationally mobilized late in the response. Surprisingly, certain transcriptionally induced mRNAs were excluded from ribosomal association after shock. Importantly, we verify, using constructs with intact 5' and 3' untranslated regions, that the observed changes in polysomal mRNA are reflected in protein levels, including cases with only translational up-regulation. Interestingly, the translational regulation of the most highly osmostress-regulated mRNAs was more strongly dependent on the stress-activated protein kinases Hog1 and Rck2 than the transcriptional regulation. Our results show the importance of translational control for fine tuning of the adaptive responses.This work was financially supported by the European Commission (QUASI; LSHG-CT2003-530203 and UNICELLSYS; LSHG-CT2007-201142) to P.S and F.P, grants from MICINN and Consolider from the Spanish government, EURYI scheme award (www.esf.org/euryi), ICREA Acadèmia (Generalitat de Catalunya) and Fundación Marcelino Botín (FMB) to F.P, and by grants from the Swedish Research Council (2007-5460) and the Swedish Cancer Fund (09-0772) to P. S

Warringer, Jonas

Hult, Malin

Regot Rodríguez de Mier, Sergi

Posas Garriga, Francesc

Sunnerhagen, Per

UPF Digital Repository

The HOG pathway dictates the short-term translational response after hyperosmotic shock

Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins including hexose transporters were enriched. Similarly, numerous mRNAs encoding cytoplasmic ribosomal proteins run counter to the overall trend of down-regulation and are instead translationally mobilized late in the response. Surprisingly, certain transcriptionally induced mRNAs were excluded from ribosomal association after shock. Importantly, we verify, using constructs with intact 5′ and 3′ untranslated regions, that the observed changes in polysomal mRNA are reflected in protein levels, including cases with only translational up-regulation. Interestingly, the translational regulation of the most highly osmostress-regulated mRNAs was more strongly dependent on the stress-activated protein kinases Hog1 and Rck2 than the transcriptional regulation. Our results show the importance of translational control for fine tuning of the adaptive responses.</jats:p

Crossref

The HOG Pathway Dictates the Short-Term Translational Response after Hyperosmotic Shock

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Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in ﬁssion yeast.

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Stressinduced map kinase Hog1 is part of transcription activation complexes.

Targeting the MEF2-like transcription factor Smp1 by the stress-activated Hog1 mitogen-activated protein kinase.

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The HOG Pathway Dictates the Short-Term Translational Response after Hyperosmotic Shock

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