Bielsa Pérez, Beatriz

Leida, C.

citaREA Repositorio Electrónico Agroalimentario

 RGC7                                           June 24-26, 2014                                         Seattle, WA, USA             98 Poster number: 53 Expression of related genes to the response of drought in Prunus rootstocks  Beatriz Bielsa1, Carmen Leida2, María José Rubio1  1Agrifood Research and Technology Centre of Aragon (CITA), Zaragoza, Spain; 2Fondazione Edmund Mach (FEM) Research and Innovation Centre Molecular Biology of Fruit Crops, S. Michele all’Adige, TN, Italy  Abstract: Climate change conditions accentuate drought since the reduction of 10% of the precipitation is translated in a reduction of 25% of the soil water. Therefore, it is indispensable the use of adapted rootstocks to water stress conditions. In order to understand their response to drought, physiological and molecular parameters of three Prunus hybrid rootstocks, the almond x peach hybrid (P. amygdalus x P. persica) ‘Garnem’, their progenies ‘P.2175’ x ‘Garnem’-3 trihybrid (P. cerasifera x [P. amygdalus x P. persica]) and ‘P.2175’ x ‘Garnem’-9 trihybrid were investigated. Plants in pots were subjected to drought conditions (35% water soil content) during one month. Subsequently, plants were submitted to re-watering period. Physiological responses were monitored through transpiration and leaf water potential showing significant differences along the experiment and among the genotypes. Gene expression analysis of four genes coding for proteins related to ABA pathway and abiotic stress were analyzed by RT-qPCR. A dehydrin (ppa005514m), a LEA protein, (ppa008651m), an A20/AN1 zinc finger (ppa012373m), and a bZIP transcription factor (ppa013046m). The profile expression of the four genes showed a correlation with physiological parameters of drought response, being higher in root than in phloem tissue. The zinc finger and bZIP transcription factors showed differences in expression relative to the target genes LEA protein and dehydrin under drought and recovery treatment, showing a regulatory response to water stress in Prunus genotypes.  Keywords: ABA, LEA protein, real-time PCR, transcription factors, water stress    

Expression of related genes to the response of drought in Prunus rootstocks: abstract

http://citarea.cita-aragon.es/citarea/bitstream/10532/2588/1/2014_151.pdf

Expression of related genes to the response of drought in Prunus rootstocks: abstract

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citaREA Repositorio Electrónico Agroalimentario