Frost, \u2707, Erik

Zavazava, Faculty Advisor, Nicholas

Digital Commons @ Illinois Wesleyan University

Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2007, 18th Annual JWP ConferenceApr 14th, 9:00 AM - 10:00 AMIdentification and Migration of Fused BM Cells In-VivoErik Frost, '07Illinois Wesleyan UniversityNicholas Zavazava, Faculty AdvisorIllinois Wesleyan UniversityFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Erik Frost, '07 and Nicholas Zavazava, Faculty Advisor, "Identification and Migration of Fused BM Cells In-Vivo" (April14, 2007). John Wesley Powell Student Research Conference. Paper 38.http://digitalcommons.iwu.edu/jwprc/2007/posters/38THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE - A PRIL 2007 Poster Presentation P35 IDENTIFICATION AND MIGRATION OF FUSED 8M CELLS IN-VIVO Erik Frost and Nicholas Zavazava* Biology Department, Illinois Wesleyan University and University of Iowa Adult hematopoietic cells cannot be re-programmed into differentiating into another lineage except in very rare circumstances. The process is very inefficient and does not yield sufficient cell numbers for any meaningful application in therapies. However, it is possible that through fusion the necessary reprogramming of cells for therapeutic use can be accompli shed. We hypothesized that transplanted BM will fuse with peripheral cells in vivo which could lead to regenerative capabil ities and altered immogenicity of these cells. BM cells from a mouse transgenic for the expression of cre recombinase were isolated and then injected intravenously into two ROSA 26 mice that were transgenic for two 10xP sites around a stop cassette in the coding region of a lacZ reporter gene (&#946;-gal). In the event of fusion the cre recombinase will excise the floxed stop cassette allowing for expression of the reporter gene. We reasoned that if cells stained positive for this reporter gene then fusion must have occurred. One mouse was sacrificed at 7 days and the other was sacrificed at 2 1  days. Cells from the liver, spleen, and BM were isolated and stained for &#946;-gal and flow cytometry was run. Results show between 1 0%- 12% of cells stained positive for &#946;-gal confirming that fusion has occurred. Other peripheral tissue was removed, frozen, and then slides were prepared using a cryostat. These sl ides were then fixed, stained for &#946;-gal, and examined using l ight microscopy. Data shows positive staining cells in many peripheral tissues confirming that fusion has occurred. These newly generated fusion cells can now be i solated and their regenerative capabilities and immogenicity determined leading to possible clinical applications. 

Identification and Migration of Fused BM Cells In-Vivo

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Identification and Migration of Fused BM Cells In-Vivo

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