수소생산성 개선 우수고세균 개발 및 실증연구

Abstract

Thermococcus onnurineus NA1 was isolated from a deep-sea hydrothermal vent. It was shown that T. onnurineus NA1 is capable of producing H2 under anaerobic growth conditions supplemented with CO, formate, or starch. The polyphasic approach by employing transcriptomic, proteomic and metabolomic tools allowed us to understand how the strain regulates the metabolism upon the presence of one carbon substrates coupled with H2 production. Currently, we are trying to enhnace H2 productivity by the combination of genetic and process engineering tools, and H2 production rate of the engineered strains was significantly enhanced on CO or by-product gas of steel-mill process. Recently, H2 production from CO has been demonstrated at the pilot scale bioreactor. The result and future prospect would be duscussed.y employing transcriptomic, proteomic and metabolomic tools allowed us to understand how the strain regulates the metabolism upon the presence of one carbon substrates coupled with H2 production. Currently, we are trying to enhnace H2 productivity by the combination of genetic and process engineering tools, and H2 production rate of the engineered strains was significantly enhanced on CO or by-product gas of steel-mill process. Recently, H2 production from CO has been demonstrated at the pilot scale bioreactor. The result and future prospect would be duscussed.2