Total knee replacement designs claim characteristic kinematic performance that is rarely assessed in patients. In the present study, in vivo kinematics of a new prosthesis design was measured during activities of daily living. This design is posterior stabilized for which spine-cam interaction coordinates free axial rotation throughout the flexion-extension arc by means of a single radius of curvature for the femoral condyles in the sagittal and frontal planes. Fifteen knees were implanted with this prosthesis, and 3D video-fluoroscopic analysis was performed at 6-month follow-up for three motor tasks. The average range of flexion was 70.1\ub0 (range: 60.1-80.2\ub0) during stair-climbing, 74.7\ub0 (64.6-84.8\ub0) during chair-rising, and 64.1\ub0 (52.9-74.3\ub0) during step-up. The corresponding average rotation on the tibial base-plate of the lines between the medial and lateral contact points was 9.4\ub0 (4.0-22.4\ub0), 11.4\ub0 (4.6-22.7\ub0), and 11.3\ub0 (5.1-18.0\ub0), respectively. The pivot point for these lines was found mostly in the central area of the base-plate. Nearly physiological range of axial rotation can be achieved at the replaced knee during activities of daily living