Hemocytes were fractionated by centrifugation on a discontinuous Percoll density
gradient from the hemolymph of Phallusia mamillata. Results obtained from microcultures of the
fractionated hemocytes, sugar-inhibition experiments, SDS-PAGE, and immunoblotting indicate that
"compartment cells" release cellular-type (CL) lectins that are specific for a-lactose and lactulose.
The released lectins have the same properties as the CL lectins that were previously isolated from
sonicated unfractionated hemocytes, but they differ in terms of some molecular and immunological
properties from the lectins (SL) purified from the serum. SLs were never found in the supernatants
from microcultures ofthe fractionated hemocytes
