M: Protein molecular weight marker; lane 1, total protein extracted from E. coli BL21 (DE3); lane 2: E. coli containing pET-24a-CTSL without IPTG induction; lane 3: E. coli containing pET-24a-CTSL with IPTG induction; lane 4: soluble protein; lane 5: inclusion bodies; lane 6: purified recombinant CTSL protein. (TIF)</p