Unrooted phylogenetic tree of CTSL and other homologs.

Abstract

The neighbor-joining algorithm constructed the tree using the Mega11 program based on the multiple sequence alignments by Clustal Omega. The branches indicate bootstrap values of 1,000 replicates (%). The scale bar corresponds to 0.05 estimated amino acid substitutions per site. The amino acid sequences of all species were obtained from the NCBI database. GenBank accession numbers are as follows: Drosophila bipectinata (XP017106640.1), Drosophila eugracilis (XP017068923.1), Drosophila serrata (XP020800944.1), Anopheles gambiae (XP001689282.1), Aedes aegypti (XP001655999.2), Culex quinquefasciatus (XP001848344.1), Macrobrachium rosenbergii (AJP62583.2), Penaeus monodon (AME17651.1), Danio rerio (CAA69623.1), and Homo sapiens (NP001244901.1).</p