APEX2 proximity labeling reveals SRRD in close proximity to intermediate filaments and regulators of IF oligomerization.

Abstract

1) HEK293Ts stably expressing SRRD-HA stained for HA and either CANX or mitochondria. B) Schematic of APEX2 proximity labeling experiment where APEX2 is fused to SRRD or to an NES control. C) Volcano plot of APEX2 proximity labeling mass spectrometry output, where fold change (x-axis) is plotted by significance (y-axis). Colored dots correspond to STRING clusters in 2E. (*) correspond to indicate functional annotations of interest highlighted in STRING cluster in 2E. D) Filtered GSEA (cellular compartment) of SRRD-APEX2 dataset. E) Clustering of top protein-protein interactions (STRING database) of top 88 proteins ranked by fold change and p-value. Clusters generated with MCL clustering and excludes proteins with no known connections and clusters with insignificant p-values. Clusters colored based on STRING annotated GO terms and proteins with functional annotations of interested are highlighted as follows: Protein-disulfide isomerases circled in pink, proteins involved in calcium binding circled in orange.</p