Loss of SRRD results in disorganized and downregulated IFs.

Abstract

A) Confocal images of indicated cells lines stained for VIM. B) Volcano plot of quantitative proteomics experiment comparing SRRD clonal KO HEK293Ts to WT HEK293Ts where fold change (x-axis) is plotted by significance (y-axis). Horizontal dashed line represents adjusted p-value cutoff of 0.05, vertical line represents fold change of -1. Orange and green colored dots correspond to STRING clusters in 3C. C) Select clusters of top depleted proteins in SRRD KO (STRING database) ranked by fold change and p-value. Clusters generated with MCL clustering and excludes proteins with insignificant p-values. Clusters colored based on STRING annotated GO terms. D) Filtered GSEA (cellular compartment) of quantitative proteomics dataset. E) Representative images of NGN2 neurons transduced with SRRD CRISPRi sgRNA or non-targeting control, stained for MAP2 and INA. F) Quantification of the area per cell covered by INA and MAP2 signal in SRRD CRISPRi and NTC control NGN2 neurons.</p