Overexpression of WWP2 promoted Hcy-induced proliferation, migration, and phenotypic transformation of VSMC.

Abstract

A. The transfection efficiency of WWP2 overexpression and interference plasmid was determined by western blot. B. Cell viability assessed using MTT assay. C. Quantification of results from EdU assays. D. Results of the scratch test. E. EdU staining diagram. Red fluorescence refers to nuclei in the proliferative period and blue fluorescence refers to nuclei in the non-proliferative period. The cell proliferation rate for each group was calculated by dividing the number of red nuclei by blue nuclei. F. Cell scratch test at 0h and 48h. G. qRT-PCR was used to detect the mRNA expressions of a-SMA, SM22a and OPN. H. Western blot was used to detect the protein expressions of a-SMA, SM22a and OPN. The experiment was performed in triplicate, and the representative images are shown.*PP# P## P<0.01 compared with the Hcy group.</p