InR manipulations in α′β′ MBn affects HAB.

Abstract

(A) Genetic expression of InRDN in α′β′ MBn via VT57244-GAL4 > UAS-InRDN increased HAB during both sated and hungry states (Satiety: P-values: 0.0003, P-values: B) Genetic expression of InRDN in γ MBn via VT44966-GAL4 > UAS-InRDN had no effect on HAB during both sated and hungry states (Satiety: P-values: 0.3221, 0.9858, 0.6006, and 0.8822 from left to right; Hunger: P-values: 0.9897, 0.6707, 0.8446, and 0.5868 from left to right). (C) Genetic expression of InRDN in γ MBn via R16A06-GAL4 > UAS-InRDN had no effect on HAB during both sated and hungry states (Satiety: P-values: 0.3765, 0.8375, 0.9045, and 0.9183 from left to right; Hunger: P-values: 0.5816, 0.8305, 0.8359, and 0.7554 from left to right). (D) Genetic expression of InRDN in αβ MBn via VT49246-GAL4 > UAS-InRDN had no effect on HAB during both sated and hungry states (Satiety: P-values: 0.9815, 0.4656, 0.9837, and 0.9286 from left to right; Hunger: P-values: 0.9478, 0.9479, 0.9743, and 0.9928 from left to right). (E) Genetic expression of InRDN in αβ MBn via C739-GAL4 > UAS-InRDN had no effect on HAB during both sated and hungry states (Satiety: P-values: 0.9846, 0.7737, 0.7182, and 0.7518 from left to right; Hunger: P-values: 0.5773, 0.6921, 0.853, and 0.4112 from left to right). (F) Permissive temperature control of Fig 2E. At permissive temperatures, there were no significant differences in HAB between tub-GAL80ts; VT30604-GAL4 > InRDN, tub-GAL80ts; VT30604-GAL4 >+, and UAS-InRDN >+ flies during sated and hungry states (Satiety: P-values: 0.4415, 0.7193, 0.7097, and 0.9129 from left to right; Hunger: P-values: 0.9994, 0.7927, 0.9944, and 0.3847 from left to right). (G) Genetic expression of InRDN in α′β′ MBn via VT30604-GAL4 > UAS-InRDN had no effect on the cold avoidance behavior during sated and hungry states (Satiety: P-values: 0.4082, 0.9822, 0.9626, and 0.7241 from left to right; Hunger: P-values: 0.5038, 0.9253, 0.7114, and 0.6802 from left to right). (H) Genetic expression of InRDN in α′β′ MBn via VT57244-GAL4 > UAS-InRDN had no effect on the cold avoidance behavior during both sated and hungry states (Satiety: P-values: 0.6194, 0.7396, 0.8875, and 0.8173 from left to right; Hunger: P-values: 0.5038, 0.5851, 0.6177, and 0.7691 from left to right). Each N represents a group of 15 flies analyzed together in the behavioral assay. The data underlying this figure can be found in S1 Data. Data are represented as mean ± SEM with dots representing individual values and analyzed by one-way ANOVA followed by Tukey’s test, *P (TIF)</p