Immunostaining with anti-Dilp2 antibody in cg-GAL4 > UAS-dilp6RNAi (dilp6RNAi), cg-GAL4 > + (Control), and cg-GAL4 > UAS-dilp6 (dilp6) flies (left panel). Quantification of anti-Dilp2 immunopositive signals in IPC during sated and hungry states (right panel). The anti-Dilp2 immunostaining signals in IPCs were normalized to the signals in the fan-shaped body (Satiety: P-values: P-values: N represents a single fly in Dilp2 immunostaining experiments. Data are represented as mean ± SEM with dots representing individual values. The data underlying this figure can be found in S1 Data. Data were analyzed by unpaired two-tailed t test. *P (TIF)</p