Exploration of the tissue architecture of IC and ID β <i>HLTF</i> KO pancreata.

Abstract

(A) Two-dimensional batch-corrected t-distributed stochastic neighbor embedding (t-SNE) visualization of the UMI counts from the entire IC vs ID dataset. (B) Pancreata clusters 16 and 18 are shown in t-SNE space. (C) Pancreatic genes insulin 1 (Ins1) and insulin 2 (Ins2) illustrated differential gene expression in IC vs ID β Hltf KO mice in t-SNE plots of clusters 16 and 18. The unique expression of immune cell markers in IC vs ID tissue is elaborated in t-SNE plots of these clusters. Slamf6 and Il2rb were added to previously identified markers for NK cells (GzmA, Klrb1b). Five markers for B cells (Pax5, Blk, Fcmr, Fcrla, Tnfrasf9) and three markers for activation of innate immunity (Bpifb1, Serpinb3a, Defb36) were unique to cluster 18.</p