Additional file 1 of Transcriptomic and metabolomic analyses revealed regulation mechanism of mixotrophic Cylindrotheca sp. glycerol utilization and biomass promotion

Abstract

Additional file 1: Figure S1. Evaluation of the promoting effects of different organic carbon sources on growth of Cylindrotheca sp. withand withoutthe presence of light. Culture was placed under 22 ± 1 °C, light intensity of 80 μmol m−2 s−1 and regime of 16 h/8 h light/darkness. Results are displayed as mean ± SD, n = 2. Figure S2. Changes in relative expression level of light-dependent genes in mixotrophic culture. Data were shown as mean ± SD, n = 9. GK: glycerol kinase; GPDH: glycerol-3-phosphate dehydrogenase [NAD]; TIM: triosephosphate isomerase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. Figure S3. Comparative transcriptomic analysis between mixotrophy and autotrophy at point B.KEGG enrichment analysis. Pathways with q < 0.05 are considered significantly enriched;Gene set enrichment analysis. KEGG pathways with q < 0.05 are considered significantly enriched and corresponding normalized enrichment scoreis noted; RES: running enrichment score; RLM: ranked list metric;Protein–protein interaction network of genes in significantly enriched KEGG pathways in GSEA analysis with top ten hub genes, evaluated by betweenness, in the center. Nos. 1–10 represent glutamate-tRNA ligase, isoleucine-tRNA ligase, phenylalanine-tRNA ligase beta subunit, tyrosine-tRNA ligase, replication protein A 32 kDa subunit B, cytoplasmic leucine-tRNA ligase, chloroplastic/mitochondrial leucine-tRNA ligase, replication protein A 70 kDa DNA-binding subunit A, DNA polymerase epsilon catalytic subunit A and DNA primase large subunit. Analysis was carried out for biological triplicates. Figure S4. The rest gene set enrichment analysis results between mixotrophy and autotrophy at pion C. Pathways with q < 0.05 are considered significantly enriched and corresponding normalized enrichment scoreis noted. Analysis was carried out for biological triplicates. RES: running enrichment score; RLM: ranked list metric. Figure S5. Orthogonal projections to latent structures-discriminant analysis and three-dimensional principal component analysis of metabolome in mixotrophy and autotrophy. Red dots and blue squares indicate biological triplicates of autotrophyas control and mixotrophy, respectively. Table S1. Primers in quantitative real-time PCR