Additional file 6 of Functional gene-guided enrichment plus in situ microsphere cultivation enables isolation of new crucial ureolytic bacteria from the rumen of cattle

Abstract

Additional file 6: Supplementary Fig. 1. Optimization of isolation methodology of functional gene-guided enrichment plus in situ microsphere cultivation for ureolytic bacteria. A. A plot of ureC-positive wells vs. dilution; B. Distribution of agarose microsphere sizes (diameter); C. Percentage of agarose microspheres with embedded bacteria at different dilutions; D. Percentage of agarose microspheres with embedded single bacteria at different dilutions; E. Percentage of agarose microspheres with PCR-detected embedded bacteria with increasing incubation time; F. Percentage of agarose microsphere with PCR-detected single bacteria with increasing incubation time